Journal: Neural Development

Article Title: Early-born neurons in type II neuroblast lineages establish a larval primordium and integrate into adult circuitry during central complex development in Drosophila

doi: 10.1186/1749-8104-8-6

Figure Lengend Snippet: Clusters of midline associated neurons and the fan-shaped body primordium are revealed by Gal4 14-94 driven labeling of dorsomedial (DM) lineages in the late larval brain. (A) Distal cell clusters corresponding to DM neuroblasts and their recently born progeny as well as a cluster of midline associated cells in close proximity to the fan-shaped body primordium are labeled by Gal4 14-94 . The dotted line indicates midline associated cells and primordium. DM1 to DM6 are indicated with numbers; lat, lateral type II lineages. Maximum projection of all stacks. (B,B’) The Gal4 14-94 -labeled fan-shaped body primordium (green) is colabeled with the neuropile marker DN-cadherin (magenta/white). (C-C”’) The Gal4 14-94 -labeled fan-shaped body primordium (green) is not colabeled with the synaptic marker Bruchpilot (magenta/white). (D,D’) The Gal4 14-94 -labeled fan-shaped body primordium (green) is colabeled with the membrane marker Neurotactin (magenta/white). (E-J) Single sections at different depths show the GAL4 14-94 -labeled cells of type II lineages (green) in a brain hemisphere colabeled for Neurotactin (magenta) revealing the relative position of the midline associated cells, the neurite fascicles of the DM lineages, and the fan-shaped body primordium (FBpr). DM1 to DM6 are indicated with numbers; lat, lateral type II lineages. Circles in (I) indicate neurite fascicles. (K-K”) GAL4 14-94 -labeled cells and projections of type II lineages (green) at the midline colabeled for DN-cadherin (magenta). The two bilateral clusters of midline-associated cells (K) give rise to four neurite fascicles per hemisphere (K’) , which project into the fan-shaped body primordium (K”). The fan-shaped body primordium is composed of four subcompartments per hemisphere (arrowheads) (K”) . FBpr, fan-shaped body primordium; dlrFB, dorsolateral root of fan-shaped body; mrFB, medial root of fan-shaped body; lrFB, lateral root of fan-shaped body; nomenclature according to . (B-D’, K-K”) are maximum intensity projections of few adjacent confocal slices. (A) and (E-K”) scale bars, 25 μm, (B-D’) scale bars, 10 μm.

Article Snippet: The following antibodies were used: rabbit anti-β-galactosidase 1:500 (55976, MP Biomedicals, Solon, OH, USA), chicken anti-GFP 1:1,000 (ab13970, Abcam, Cambridge, UK), mouse anti-Neurotactin 1:20 (BP106, Developmental Studies Hybridoma Bank (DSHB), Iowa city, Iowa, USA), rat anti-Elav 1:20 (7E8A10, DSHB), rabbit anti-Repo 1:400 (kindly provided by Veronica Rodrigues), rat anti-DN-cadherin 1:10 (DN-EX #8, DSHB), mouse anti-Nc82 1:20 (Bruchpilot, DSHB), rabbit anti-RFP 1:200 (ab62341, Abcam, Cambridge, UK).

Techniques: Labeling, Marker

Journal: Science signaling

Article Title: Designer high-density lipoprotein particles enhance endothelial barrier function and suppress inflammation

doi: 10.1126/scisignal.adg9256

Figure Lengend Snippet: (A) Side view of A1M-S1P complex with and without the phospholipids, highlighting the S1P binding pocket in both the ApoM-S1P and ApoA1-fused systems. Monomer 1 - ApoA1 in sienna, linker in brown, and ApoM in beige. Monomer 2 - ApoA1 in sage, linker in turquoise, and ApoM in teal. Detail of the binding pocket in A1M-S1P and ApoM-S1P are shown in the right panel. (B) Projection of protein atoms along the top two dominant eigenvectors (eigenvector 1 - PC1 and eigenvector 2 – PC2) is responsible for over 40% of the dominant collective variances in the structure set, generating 5 clusters for which the three-dimensional structure of the most representative member of each cluster is shown. Permanence times: cluster 1 – 9.6%, cluster 2 – 52.9%, cluster 3 – 18.7%, cluster 4 – 12.2%, cluster 5 – 6.5%. (C) Distribution of the proportion of variance between the PCs.

Article Snippet: The A1M fusion was constructed using plasmids for murine ApoA1 (MR203500) and murine ApoM (MR201811) obtained from OriGene.

Techniques: Binding Assay

Journal: Science signaling

Article Title: Designer high-density lipoprotein particles enhance endothelial barrier function and suppress inflammation

doi: 10.1126/scisignal.adg9256

Figure Lengend Snippet: (A) HMEC-1 cells expressing an NF-κB-luciferase reporter were assayed for TNFα-induced NF-κB reporter activity in the presence of ApoA1, A1M, A1M-S1P, and ApoM-Fc-S1P (N = 3 biological replicates per group). Data are presented as means + S.D. **P < 0.01, ***P < 0.001, ****P < 0.0001 by ordinary two-way ANOVA with Tukey’s multiple comparisons test. TNFα is the reference group. (B) HUVECs were starved for 1 h, pre-treated for 10 minutes with ApoM-Fc-S1P (100 nM), iloprost (200 nM), both ApoM-Fc-S1P and iloprost, A1M (200 μg/mL), A1M-S1P (200 μg/mL), or A1M-iloprost (200 μg/mL) and induced with TNFα (10 ng/mL) for 5 hours. Lysates were subjected to immunoblot analysis for ICAM-1. Quantification of immunoblots was analyzed from 3 biological replicates per group. Data are presented as means ± S.D. *P < 0.05, **P < 0.01, ****P < 0.0001 by ANOVA with post-hoc Holm-Sidak’s multiple comparisons test. (C) Cholesterol efflux in response to human HDL (hHDL), human ApoA1 protein (hApoA1), and bacterial A1M (bA1M) in PMA-induced THP-1 cells (N ≥ 5 independent experiments). Data are presented as means + S.D. *P < 0.05, ***P < 0.001, ****P < 0.0001 by one-way ANOVA with Dunnett’s multiple comparisons test.

Article Snippet: The A1M fusion was constructed using plasmids for murine ApoA1 (MR203500) and murine ApoM (MR201811) obtained from OriGene.

Techniques: Expressing, Luciferase, Activity Assay, Western Blot